A Fully Automated Chemical Separation and Purification System and Its Application to Isotope Analysis

ZHU Zhi-yong; ZHU Xiang-kun; YANG Tao

doi:10.15898/j.cnki.11-2131/td.201908120123

Rock and Mineral Analysis > 2020 > 39(3): 384-390. > DOI: 10.15898/j.cnki.11-2131/td.201908120123

ZHU Zhi-yong, ZHU Xiang-kun, YANG Tao. A Fully Automated Chemical Separation and Purification System and Its Application to Isotope Analysis[J]. Rock and Mineral Analysis, 2020, 39(3): 384-390. DOI: 10.15898/j.cnki.11-2131/td.201908120123

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A Fully Automated Chemical Separation and Purification System and Its Application to Isotope Analysis

1.
Institute of Geology, Chinese Academy of Geological Sciences, Beijing 100037, China
2.
School of Earth Sciences and Engineering, Nanjing University, Nanjing 210093, China

More Information

Received Date: August 11, 2019
Revised Date: November 25, 2019
Accepted Date: January 14, 2020
Published Date: April 30, 2020

Abstract

HIGHLIGHTS
(1) The automated chemical separation and purification system could minimize the tailing effect of ions on the chromatographic column.

(2) The combined chromatographic columns were applied to the automated chemical separation and purification system.

(3) Reversed elution was achieved with the automated chemical separation and purification system.

ABSTRACT

BACKGROUNDCritical problems such as ion diffusion and uncontrollable flow rate exist when applying the traditional natural flow chromatography column method to isotope analysis. The proposed automated chemical purification system improves the efficiency of isotope analysis, and reduces the time required for element purification.
OBJECTIVESTo develop an automated chemical separation and purification system.
METHODSThe automated chemical separation and purification system was composed of multi-way valves, plunger pump, autosampler, etc. All of the components were connected using a computer and each of them can be controlled independently. By combining two chromatographic columns multiple functions can be achieved, including reversed elution, which cannot be achieved by the traditional natural flow chromatography column method. In order to investigate the performance of the automated chemical separation and purification system, fresh seawater was purified with the proposed system and the traditional method simultaneously.
RESULTSThe elution peak of boron had a width of 2.5mL to 3.0mL for the traditional method, but only ca. 1.0mL for the proposed system, if the loading volume of seawater was 1.0mL. The flow rate of the elution depended on the acidity using the traditional natural flow chromatography column method. However, the flow rate could be strictly controlled with the automated chemical separation and purification system.
CONCLUSIONSThe precisely controlled flow rate of the system makes an easier prediction of the elution time and elution peak. The reversed column technology of the system can be applied to the separation and purification of elements for isotope analysis.
- automatic separation and purification,
- isotope analysis,
- combined chemical columns,
- partitioning coefficient

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References (32)

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