Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method

XU Hong-na; JIN Li-guo; YOU Li-mei; CHENG Yan

doi:10.15898/j.cnki.11-2131/td.201908090118

Rock and Mineral Analysis > 2020 > 39(5): 785-792. > DOI: 10.15898/j.cnki.11-2131/td.201908090118

XU Hong-na, JIN Li-guo, YOU Li-mei, CHENG Yan. Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method[J]. Rock and Mineral Analysis, 2020, 39(5): 785-792. DOI: 10.15898/j.cnki.11-2131/td.201908090118

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Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method

1.
Mudanjiang Medical University, Mudanjiang 157011, China
2.
The First Geological Prospecting Institute of Heilongjiang Province, Mudanjiang 157011, China

More Information

Received Date: August 08, 2019
Revised Date: December 10, 2019
Accepted Date: April 15, 2020
Published Date: August 31, 2020

Abstract

HIGHLIGHTS
(1) The derivative spectrophotometric method can be used for resolving overlapped peaks, and thus eliminating the interference of Cr(Ⅲ) on Cr(Ⅵ).

(2) When the derivative interval factor was 10nm, the first derivative value of absorption was the most suitable.

(3) Cr(Ⅲ)-EDTA coordination compound has high stability, and its absorbance value did not change within 3 hours.

ABSTRACT

OBJECTIVESChromium is usually found as Cr(Ⅲ) and Cr(Ⅵ) in environmental water samples. The different valences of chromium produce different physiological function, thus it is necessary to analyze the different valences of chromium accurately. At present, the contents of Cr(Ⅲ) and Cr(Ⅵ) are mostly determined after separation or the content of Cr(Ⅲ) or Cr(Ⅵ) is measured first, then the total content of Cr is determined after oxidation or reduction. The content of another valence of chromium is then calculated by the subtraction method. The available method needs a complex procedure. Moreover, the valence of Cr is easily modified during the sample treatment, resulting in large error and low precision.
OBJECTIVESTo find a simple and accurate method for determination of Cr(Ⅲ) and Cr(Ⅵ).
METHODSThe first derivative spectrophotometric method was used for simultaneously determining of Cr(Ⅲ) and Cr(Ⅵ), which eliminated the interference of Cr(Ⅲ) on Cr(Ⅵ).
RESULTSChromogenic reagent EDTA-2Na was added to the mixed water sample in a 70℃water-bath at pH 3-3.5 for 15min, and the absorbance was measured. When the derivative interval factor was 10nm, the first derivative value of absorption for Cr(Ⅵ) was the maximum at 330nm wavelength and the value for Cr(Ⅲ)-EDTA was zero. The concentration of Cr(Ⅵ) can be obtained by the first derivative spectrophotometric method, whereas Cr(Ⅲ) can be determined directly at the maximum wavelength of 543nm. Under the optimal conditions, the concentration range was 0-100mg/L for Cr(Ⅵ) and 0-120mg/L for Cr(Ⅲ). The equation of linear regression for Cr(Ⅲ) was A=0.0036ρ-0.0002 (r²=0.9999), for Cr(Ⅵ) was D=0.00072ρ-0.00013 (r²=0.9996). and the limit of detection was 0.005mg/L for Cr(Ⅵ) and 0.006mg/L for Cr(Ⅲ). The recoveries for Cr(Ⅲ) and Cr(Ⅵ) were 97.8%-102.6%.
ConclusionThe method meets the requirements for analyzing waste water.
- water sample,
- Cr(Ⅲ),
- Cr(Ⅵ),
- simultaneous determination,
- derivative interval factor,
- first derivative spectrophotometry

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References (35)

References

王蓉, 张丽萍, 邹时英.偶氮胂Ⅲ褪色光度法测定土壤中的铬[J].岩矿测试, 2011, 30(2):230-232. http://www.ykcs.ac.cn/article/id/ykcs_20110224

Wang R, Zhang L P, Zou S Y.Determination of chromium in soil samples by arsenazo Ⅲ fading spectrophotometry[J].Rock and Mineral Analysis, 2011, 30(2):230-232. http://www.ykcs.ac.cn/article/id/ykcs_20110224

Chen S J, Zhang X S, Yu L Y, et al.Simultaneous determination of Cr(Ⅲ) and Cr(Ⅵ) in tannery wastewater using low pressure ion chromatography combined with flow injection spectrophotometry[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2012, 88:49-55. doi: 10.1016/j.saa.2011.11.053

Ouejhani A, Hellal F, Dachraoui M, et al.Application of doehlert matrix to the study of electrochemical oxidation of Cr(Ⅲ) to Cr(Ⅵ) in order to recover chromium from wastewater tanning baths[J].Journal of Hazardous Materials, 2008, 157(2/3):423-431. http://www.sciencedirect.com/science/article/pii/S0304389408000447

Tardif S, Cipullo S, Sø H U, et al.Factors governing the solid phase distribution of Cr, Cu and As in contaminated soil after 40 years of ageing[J].Science of the Total Environment, 2019, 652:744-754. doi: 10.1016/j.scitotenv.2018.10.244

Diao Z H, Du J J, Jiang D, et al.Insights into the simultaneous removal of Cr⁶⁺ and Pb²⁺ by a novel sewage sludge derived biochar immobilized nanoscale zero valent iron:Coexistence effect and mechanism[J].Science of the Total Environment, 2018, 642:505-515. doi: 10.1016/j.scitotenv.2018.06.093

张杰芳, 闫玉乐, 夏承莉, 等.微波碱消解-电感耦合等离子体发射光谱法测定煤灰中的六价铬[J].岩矿测试, 2017, 36(1):45-51. doi: 10.15898/j.cnki.11-2131/td.2017.01.007

Zhang J F, Yan Y L, Xia C L, et al. Determination of Cr(Ⅵ) in coal ash by microwave alkaline digestion and inductively coupled plasma-optical emission spectrometry[J].Rock and Mineral Analysis, 2017, 36(1):45-51. doi: 10.15898/j.cnki.11-2131/td.2017.01.007

李冰茹, 杜远芳, 王北洪, 等.食品中总铬和铬形态分析的前处理技术概述[J].食品安全质量检测学报, 2018, 9(9):2056-2062. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spaqzljcjs201809013

Li B R, Du Y F, Wang B H, et al.Research progress on the pretreatment techniques in the analysis of total chromium and speciation chromium in food[J].Journal of Food Safety and Quality, 2018, 9(9):2056-2062. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spaqzljcjs201809013

Economou-Eliopoulos M, Megremi Ⅰ, Vasilatos C. Geochemical constraints on the sources of Cr(Ⅵ) contamination in waters of Messapia (Central Evia) Basin[J].Applied Geochemistry, 2017, 84:13-25. doi: 10.1016/j.apgeochem.2017.05.015

安茂国, 赵庆令, 谭现锋, 等.化学还原-稳定化联合修复铬污染场地土壤的效果研究[J].岩矿测试, 2019, 38(2):204-211. doi: 10.15898/j.cnki.11-2131/td.201806040068

An M G, Zhao Q L, Tan X F, et al.Research on the effect of chemical reduction-stabilization combined remediation of Cr-contaminated soil[J].Rock and Mineral Analysis, 2019, 38(2):204-211. doi: 10.15898/j.cnki.11-2131/td.201806040068

Mulware S J.Trace elements and carcinogenicity:A subject in review[J].Biotechnology, 2013, 3(2):85-96. doi: 10.1007/s13205-012-0072-6

Clemention M, Shi X L, Zhang Z.Oxidative stress and metabolic reprogramming in Cr(Ⅵ) carcinogenesis[J].Current Opinion in Toxicology, 2018, 8:20-27. doi: 10.1016/j.cotox.2017.11.015

陈思涵, 彭璨, 陈云生, 等.分光光度法测定胶囊中Cr(Ⅲ)和Cr(Ⅵ)[J].中国卫生检验杂志, 2015, 25(1):18-20. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgwsjyzz201501005

Chen S H, Peng C, Chen Y S, et al.Detection of Cr(Ⅲ) and Cr(Ⅵ) in capsule with spectrophotometry[J].Chinese Journal of Health Laboratory Technology, 2015, 25(1):18-20. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgwsjyzz201501005

张冀飞, 高欣, 杨晓兵.高效液相色谱-电感耦合等离子体质谱联用测定儿童玩具中痕量铬(Ⅲ)与铬(Ⅵ)[J].分析测试学报, 2015, 34(2):232-236. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxcsxb201502019

Zhang J F, Gao X, Yang X B.Determination of chromium(Ⅲ) and chromium(Ⅵ) in children's toys using high performance liquid chromatography-inductively coupled plasma mass spectrometry[J].Journal of Instrumental Analysis, 2015, 34(2):232-236. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxcsxb201502019

林莉, 郑翊, 卫碧文, 等.IC-ICP-MS联用法测定玩具材料中可迁移的六价铬与三价铬[J].分析试验室, 2013, 32(8):82-85. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxsys201308018

Lin L, Zheng Y, Wei B W, et al.Simultaneous determination of migratory chromium species in toy materials by ion chromatography and ICP-MS[J].Chinese Journal of Analysis Laboratory, 2013, 32(8):82-85. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxsys201308018

闫美, 谢晨星, 朱智惠, 等.保健食品中三价铬与六价铬的分离与测定[J].食品研究与开发, 2016, 37(7):171-175. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spyjykf201607042

Yan M, Xie C X, Zhu Z H, et al.The research on the conditions of hexavalent chromium convert to trivalent chromium in healthy food[J].Food Research and Development, 2016, 37(7):171-175. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spyjykf201607042

刘明理, 曹进, 丁宏, 等.液相色谱-质谱法测定保健食品中的三价铬及六价铬含量[J].食品安全质量检测学报, 2017, 8(7):2465-2470. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spaqzljcjs201707016

Liu M L, Cao J, Ding H, et al.Determination of trivalent and hexavalent chromium in healthy food by liquid chromatography-mass spectrometry[J].Journal of Food Safety and Quality, 2017, 8(7):2465-2470. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spaqzljcjs201707016

庞艳华, 刘名扬, 刘淑艳, 等.反相离子对色谱-电感耦合等离子体质谱法测定化妆品中不同形态的铬[J].色谱, 2011, 29(10):1027-1030. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=sp201110014

Pang Y H, Liu M Y, Liu S Y, et al.Simultaneous determination of chromium speciation in cosmetics using reversed-phase ion-pair chromatography-inductively coupled plasma mass spectrometry[J].Chinese Journal of Chromatography, 2011, 29(10):1027-1030. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=sp201110014

吴思霖, 王欣美, 潘晨, 等.高效液相色谱-电感耦合等离子体质谱联用测定化妆品中六价铬与三价铬[J].分析测试学报, 2019, 38(6):724-727. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxcsxb201906015

Wu S L, Wang X M, Pan C, et al.Determination of chromium(Ⅵ) and chromium(Ⅲ) in cosmetics by high performance liquid chromatography-inductively coupled plasma mass spectrometry[J].Journal of Instrumental Analysis, 2019, 38(6):724-727. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxcsxb201906015

张保科, 王蕾, 马生凤.电感耦合等离子体质谱法测定含气天然矿泉水中的铬[J].岩矿测试, 2013, 32(4):568-571. http://www.ykcs.ac.cn/article/id/f4ece52c-7679-4883-a2ff-e57adb9b0536

Zhang B K, Wang L, Ma S F.Quantification of Cr in natural sparkling mineral waters by inductively coupled plasma-mass spectrometry[J].Rock and Mineral Analysis, 2013, 32(4):568-571. http://www.ykcs.ac.cn/article/id/f4ece52c-7679-4883-a2ff-e57adb9b0536

邢夏, 徐进力, 何晓辉, 等.石墨炉原子吸收光谱法测定地球化学样品中微量铬[J].岩矿测试, 2011, 30(3):333-336. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ykcs201103019

Xing X, Xu J L, He X H, et al.Determination of trace chromium in geochemical samples by graphite furnace atomic absorption spectrometry[J].Rock and Mineral Analysis, 2011, 30(3):333-336. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ykcs201103019

Sadeghi S, Moghaddam A Z.Chromium speciation using task specific ionic liquid/aqueous phase biphasic system combined with flame atomic absorption spectrometry[J].Journal of Molecular Liquids, 2016, 221:798-804. doi: 10.1016/j.molliq.2016.06.056

卢菊生, 徐佳佳, 田久英, 等.微乳相萃取分离富集-原子吸收光谱法分析铬形态[J].应用化学, 2010, 27(10):1230-1234. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yyhx201010022

Lu J S, Xu J J, Tian J Y, et al.Speciation determination of chromium by atomic absorption spectrometry with separation of microemulsion extraction[J].Chinese Journal of Applied Chemistry, 2010, 27(10):1230-1234. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yyhx201010022

Tafti E N, Dadfarnia S, Shabani A M H.Supramolecular dispersive liquid-liquid microextraction-based solidification of floating organic drops combined with electrothermal atomic absorption spectrometry for determination of chromium species[J].International Journal of Environmental Analytical Chemistry, 2017, 97(5):444-445. doi: 10.1080/03067319.2017.1321743

Monasterio R P, Lascalea G E, Martínez L D, et al. Determination of Cr(Ⅵ) and Cr(Ⅲ) species in parenteral solutions using a nanostructured material packed-microcolumn and electrothermal atomic absorption spectrometry[J].Journal of Trace Elements in Medicine and Biology, 2009, 23(3):157-166. doi: 10.1016/j.jtemb.2009.03.002

Lee C F, Chen B H, Huang Y L.Determining Cr(Ⅲ) and Cr(Ⅵ) in urine using a flow injection on-line sorption separation system coupled with electrothermal atomic absorption spectrometry and a UV/nano-Au/TiO₂ photocatalysis reduction device[J].Talanta, 2008, 77(2):546-550. doi: 10.1016/j.talanta.2008.03.018

徐红纳, 王英滨.双波长分光光度法同时测定水样中的Cr(Ⅲ)和Cr(Ⅵ)[J].分析试验室, 2008, 27(5):34-37. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxsys200805009

Xu H N, Wang Y B.Simultaneous determination of chromium(Ⅲ) and chromium(Ⅵ) by dual-wavelength spectrophotometry[J].Chinese Journal of Analysis Laboratory, 2008, 27(5):34-37. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxsys200805009

Hackbarth F Ⅴ, Maass D, Souza A A U, et al.Removal of hexavalent chromium from electroplating wastewaters using marine macroalga Pelvetia canaliculata as natural electron donor[J].Chemical Engineering Journal, 2016, 290:477-489. doi: 10.1016/j.cej.2016.01.070

Kim J S, Choi Y R, Kim Y S, et al.Determination of hexavalent chromium (Cr(Ⅵ)) in plastics using organic-assisted alkaline extraction[J].Analytica Chimica Acta, 2011, 690(2):182-189. doi: 10.1016/j.aca.2011.01.060

Abadi M D M, Chamsaz M, Arbab-Zavar M H, et al.Supramolecular dispersive liquid-liquid microextraction-based solidification of floating organic drops for speciation and spectrophotometric determination of chromium in real samples[J].Analytical Methods, 2013, 5(12):2971-2977. doi: 10.1039/c3ay00036b

孙克强, 王京力, 李浩洋, 等.离子色谱法测定玩具中三价铬和六价铬的含量[J].理化检验(化学分册), 2017, 53(9):1099-1102. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=lhjy-hx201709025

Sun K Q, Wang J L, Li H Y, et al.Determination of chromium(Ⅲ) and chromium(Ⅵ) in toys by ion chromatography[J].Physical Testing and Chemical Analysis (Part B:Chemical Analysis), 2017, 53(9):1099-1102. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=lhjy-hx201709025

巢静波, 史乃捷, 陈扬, 等.衍生液注入控制-离子色谱法同时测定环境水样中的三价铬和六价铬[J].环境化学, 2016, 35(1):67-74. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hjhx201601009

Chao J B, Shi N J, Chen Y, et al.Simultaneous determination of trivalent and hexavalent chromium in environmental waters by ion chromatography with derivatization reagent injection-control technique[J].Environmental Chemistry, 2016, 35(1):67-74. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hjhx201601009

Araujo-Barbosa U, Pena-Vazquez E, Barciela- Alonso M C, et al.Simultaneous determination and speciation analysis of arsenic and chromium in iron supplements used for iron-deficiency anemia treatment by HPLC-ICP-MS[J].Talanta, 2017, 170:523-529. doi: 10.1016/j.talanta.2017.04.034

古君平, 施文庄, 刘殷, 等.高效液相色谱-电感耦合等离子体质谱法同时测定烟用接装纸中三价铬与六价铬的含量[J].理化检验(化学分册), 2017, 53(1):17-21. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=lhjy-hx201701004

Gu J P, Shi W Z, Liu Y, et al.Simultaneous determination of chromium(Ⅲ) and chromium(Ⅵ) in cigarette tipping paper by HPLC-ICP-MS[J].Physical Testing and Chemical Analysis (Part B:Chemical Analysis), 2017, 53(1):17-21. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=lhjy-hx201701004

周波林.一阶导数紫外光谱法测定人血浆中地西泮的血药浓度[J].中国医药导报, 2014, 11(20):16-19. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yycyzx201420006

Zhou B L.Determination of diazepam in human plasma by first order derivative spectrophotometry[J].China Medical Herald, 2014, 11(20):16-19. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yycyzx201420006

王华建, 黎艳红, 丰伟悦, 等.反相离子对色谱-电感耦合等离子体质谱联用技术测定水中痕量Cr(Ⅲ)与Cr(Ⅵ)[J].分析化学, 2009, 37(3):443-436. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx200903025

Wang H J, Li Y H, Feng W Y, et al.Simultaneous determination of trace Cr(Ⅲ) and Cr(Ⅵ) in water using ion-pairing reversed phase chromatography-inductively coupled plasma mass spectrometry[J].Chinese Journal of Analytical Chemistry, 2009, 37(3):443-436. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx200903025

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4.	李希冉，郭梦晗，李欣怡，祁义函，赵霞. 水环境四环素类抗生素降解技术研究进展. 化学工程师. 2024(05): 69-72+102 .
5.	张译文，段明杰，罗锦秋，吕冬梅. 我国水环境抗生素污染研究综述与对策建议. 海峡科学. 2024(03): 84-90 .
6.	高川子，廖浩麟，王毅博，郑一，郑春苗，裘文慧. 药物及个人护理用品的生态毒理. 化学进展. 2024(09): 1363-1379 .
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Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method

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