Determination of Three Nitrophenol Compounds in Soil by Ultrasonic Extraction-High Performance Liquid Chromatography-Mass Spectrometry

QIAO Songfen; QIN Chong; LIU Aiqin; AN Caixiu; LIU An; YANG Lijuan; SUN Kaixi; RAN Zhuo

doi:10.15898/j.ykcs.202303170036

Rock and Mineral Analysis > 2024 > 43(3): 501-508. > DOI: 10.15898/j.ykcs.202303170036

QIAO Songfen，QIN Chong，LIU Aiqin，et al. Determination of Three Nitrophenol Compounds in Soil by Ultrasonic Extraction-High Performance Liquid Chromatography-Mass Spectrometry[J]. Rock and Mineral Analysis，2024，43（3）：501−508. DOI: 10.15898/j.ykcs.202303170036

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Determination of Three Nitrophenol Compounds in Soil by Ultrasonic Extraction-High Performance Liquid Chromatography-Mass Spectrometry

Hebei Research Center for Geoanalysis, Hebei Key Laboratory of Mineral Resources and Ecological Environment Monitoring, Baoding 071052, China

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Received Date: March 16, 2023
Revised Date: June 03, 2023
Accepted Date: August 06, 2023
Available Online: December 07, 2023

Abstract

ABSTRACT

As important organic chemical raw materials, nitrophenol compounds have high toxicity and carcinogenicity, which can be transmitted into soil through air and water, causing pollution and endangering human health. In the past, nitrophenol compounds in soil were usually determined by gas chromatography and gas chromatography-mass spectrometry. The derivatization was usually required to improve the sensitivity, which was time-consuming and cumbersome. To achieve a rapid and accurate analysis of the three nitrophenol compounds in soil, a method of ultrasonic extraction-high performance liquid chromatography-mass spectrometry (HPLC-MS) was established. The nitrophenol compounds in soil were extracted by ultrasonic extraction with dichloromethane/n-hexane (2∶1, V/V), and a strong alkaline aqueous solution (pH>12) was added to the ultrasonic extraction solution to remove the underlying organic phase. After the aqueous solution was adjusted to acidity (pH<2), three nitrophenol compounds were extracted with dichloromethane-ethyl acetate (4∶1, V/V). The extraction solution was concentrated and diluted to 10.0mL with 10% acetonitrile aqueous solution, and then determined by HPLC-MS. The RSDs of three nitrophenol compounds were less than 10.0%, detection limits were 0.1−0.2μg/kg, and the recoveries were 61.7%−90.8%. This method has the advantages of simple pretreatment and low detection limit, and can be applied to the determination and evaluation of nitrophenol compounds in soil.
- nitrophenol compounds,
- soil,
- ultra-sonic extraction,
- dichloromethane n-hexane,
- high performance liquid chromatography-mass spectrometry

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