Spectrophotometric Determination of Available Phosphorus in Soil with Bromophenol Blue as an Acid-base Indicator

YU Tingting; ZHU Yun; GUO Lin

doi:10.15898/j.cnki.11-2131/td.202203190056

Rock and Mineral Analysis > 2023 > 42(1): 213-219. > DOI: 10.15898/j.cnki.11-2131/td.202203190056

YU Tingting, ZHU Yun, GUO Lin. Spectrophotometric Determination of Available Phosphorus in Soil with Bromophenol Blue as an Acid-base Indicator[J]. Rock and Mineral Analysis, 2023, 42(1): 213-219. DOI: 10.15898/j.cnki.11-2131/td.202203190056

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Spectrophotometric Determination of Available Phosphorus in Soil with Bromophenol Blue as an Acid-base Indicator

National Research Center for Geoanalysis, Beijing 100037, China

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Received Date: March 18, 2022
Revised Date: May 06, 2022
Accepted Date: June 24, 2022
Available Online: December 13, 2022

Abstract

HIGHLIGHTS
(1) Using bromophenol blue as an acid-base indicator was first proposed in the determination of available phosphorus in soil.

(2) Bromophenol blue showed more obvious mutation as a better acid-base indicator than dinitrophenol in this method.

(3) Extraction time, soil-liquid ratio, acidity of the extraction, and other experimental conditions were studied.

ABSTRACT

BACKGROUND
Standard and common methods for the determination of available phosphorus in soil are usually spectrophotometry methods. Before measurement, the sample is extracted by an acid or base solution, and the pH of the extracted liquid is regulated. The existing methods include complex operation steps such as filtration, and the acid-base indicator dinitrophenol is highly toxic. Most of all, the color of dinitrophenol is easily altered.
OBJECTIVES
To optimize the existing method and improve detection efficiency, and then use the method for soil available state analysis and soil evaluation.
METHODS
Different experimental conditions such as extraction time, acidity of the extraction and acid-base indicator type were compared. Centrifugal separation was used instead of filtration, and bromophenol blue was first used as the acid-base indicator. Detection results of bromophenol blue and dinitrophenol were compared.
RESULTS
Comparing the detection results of the bromophenol blue indicator and dinitrophenol indicator, the absorbance, accuracy of national standard substance and unknown sample detection results were completely consistent. With bromophenol blue as the acid-base indicator, color mutation was obvious without any interference of bottom color.
CONCLUSIONS
Bromophenol blue has low toxicity with accurate detection results. This improved method is appropriate for rapid determination of available phosphorus in acid, neutral and carbonate soils.
- soil,
- available phosphorus,
- spectrophotometry,
- acid-base indicator,
- dinitrophenol,
- bromophenol blue,
- experimental condition optimization

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References

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Spectrophotometric Determination of Available Phosphorus in Soil with Bromophenol Blue as an Acid-base Indicator

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