Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry

YING Jiaolong; QIN Xiaopeng; LANG Hang; GUO Jianyi; XIONG Ling; ZHANG Zhanhao; LIU Fei

doi:10.15898/j.cnki.11-2131/td.202111060168

Rock and Mineral Analysis > 2022 > 41(3): 394-403. > DOI: 10.15898/j.cnki.11-2131/td.202111060168

YING Jiaolong, QIN Xiaopeng, LANG Hang, GUO Jianyi, XIONG Ling, ZHANG Zhanhao, LIU Fei. Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry[J]. Rock and Mineral Analysis, 2022, 41(3): 394-403. DOI: 10.15898/j.cnki.11-2131/td.202111060168

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Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry

1.
Beijing Key Laboratory of Water Resources and Environmental Engineering, School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing 100083, China
2.
Technical Centre for Soil, Agricultural and Rural Ecology and Environment, Ministry of Ecology and Environment, Beijing 100012, China
3.
China Institute of Water Resources and Hydropower Research, Beijing 100038, China
4.
Yongding Middle School Affiliated to Capital Normal University, Beijing 102308, China

More Information

Received Date: November 05, 2021
Revised Date: December 22, 2021
Accepted Date: January 26, 2022
Available Online: July 28, 2022

Abstract

HIGHLIGHTS
(1) A method for simultaneous determination of 37 antibiotics by ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) was established, which can be applied to the detection of antibiotics in surface water and groundwater simultaneously.

(2) The method was applied to the detection of antibiotics in surface water of the Yongding River, and surface water and groundwater of the Chaobai River in Beijing, whose antibiotics were detected in different levels.

(3) The concentration of antibiotics near the sewage treatment plant was relatively high.

ABSTRACT

BACKGROUND
Currently, the environmental detection of antibiotics is receiving extensive attention, but there is still a lack of methods that can simultaneously analyze seven categories of antibiotics in surface water and groundwater.
OBJECTIVES
To establish a method that can detect thirty-seven antibiotics (15 sulfonamides, 2 tetracyclines, 3 macrolides, 8 quinolones, 2 chloramphenicols, 2 β-lactams and 5 other kinds) simultaneously.
METHODS
Solid phase extraction coupled with ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) was used to quantify the concentration of antibiotics.
RESULTS
The method had good sensitivity and enrichment effect for the surface water and groundwater, the recoveries ranged from 60% to 130%, the detection limits ranged from 0.6 to 10.6ng/L. The method was applied to the determination of antibiotics in surface water of the Yongding and Chaobai Rivers, and groundwater of the Chaobai River in Beijing. Sulfonamides, quinolones and macrolides were the main antibiotics in the surface water of the Yongding River, and the detection rates were 88.9%, 55.6% and 33.3%, respectively. The highest concentration of floxacin was 111.9ng/L. Sulfonamides, quinolones and chloramphenicols were the main antibiotics in the surface water of the Chaobai River with detection rates of 100%. The highest concentration of tilmicosin was 71.6ng/L. Sulfonamides, quinolones and β-lactams antibiotics were the main antibiotics in the groundwater of the Chaobai River Basin, and the detection rates of were 66.7%, 55.6% and 22.2%, respectively. The highest concentration of sulfadiazine was 69.3ng/L. In both surface water and groundwater, the detection rate and concentration near the sewage treatment plant were significantly higher than those at other points.
CONCLUSIONS
The method established in this study is simple, rapid and accurate, which can be used for the simultaneous determination of 37 antibiotics in seven categories in surface water and groundwater. It provides antibiotic analysis method support for surface water and groundwater investigation, and provides a scientific basis for rational utilization of water resources and management of new pollutants in water.
- antibiotic,
- ultra performance liquid chromatography-triple quadrupole-mass spectrometry,
- groundwater,
- surface water,
- Beijing City

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References (29)

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Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry