Determination of Total Nitrogen in Plant Fruits by Graphite Digestion Apparatus and Automatic Azotometer

WU Gang; ZHANG Zhao-fa; SONG Fan; LIU Xian; MA Hui-chun; PANG Xue-min; ZHANG Jia-lin; LIU Yan-yun

doi:10.15898/j.cnki.11-2131/td.201903260037

Rock and Mineral Analysis > 2020 > 39(2): 311-317. > DOI: 10.15898/j.cnki.11-2131/td.201903260037

WU Gang, ZHANG Zhao-fa, SONG Fan, LIU Xian, MA Hui-chun, PANG Xue-min, ZHANG Jia-lin, LIU Yan-yun. Determination of Total Nitrogen in Plant Fruits by Graphite Digestion Apparatus and Automatic Azotometer[J]. Rock and Mineral Analysis, 2020, 39(2): 311-317. DOI: 10.15898/j.cnki.11-2131/td.201903260037

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Determination of Total Nitrogen in Plant Fruits by Graphite Digestion Apparatus and Automatic Azotometer

Hebei Research Center for Geoanalysis, Baoding 071000, China

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Received Date: March 25, 2019
Revised Date: June 03, 2019
Accepted Date: July 15, 2019
Published Date: February 29, 2020

Abstract

HIGHLIGHTS
(1) The problems of sample splash and white smoke escape during the pretreatment of total nitrogen analysis of plant fruits were solved.

(2) The graphite digestion apparatus was combined with the automatic azotometer organically.

(3) The application of this method in the analysis of total nitrogen in plant fruits has improved work efficiency.

ABSTRACT

BACKGROUNDBy measuring the nitrogen content in plant fruits, the growth status of plants can be grasped and important information for improving the yield of high-quality fruits can be provided. At present, the traditional Kjeldahl method is used to determine nitrogen in plant fruits. The main steps are digestion, distillation and titration. The digestion time of traditional method is 90 minutes, and the titration and distillation time is 10-20 minutes. The determination process is complicated and produces errors, which is not suitable for the analysis of large amounts of plant fruits.
OBJECTIVESTo shorten the analysis and determination time of total nitrogen in plant fruit samples, improve work efficiency, avoid artificial errors, and solve the problems of sample splashing and white smoke escaping during digestion.
METHODSA graphite digestion instrument was used for digestion. Concentrated sulfuric acid and a catalyst were added, the reflux plug was capped, and an automatic nitrogen determination instrument was used for analysis.
RESULTSThe sample did not splash and the white smoke did not escape. The digestion time was 50 minutes and the distillation time was 3 minutes. The reagent consumption was reduced by 2mL. The relative error and relative standard deviation were less than 5%.
CONCLUSIONSThe established method meets the needs of the laboratory to quickly and accurately detect total nitrogen in large amounts of plant samples. It has been applied in the detailed survey project of the China geological survey bidding project and the detailed investigation of the soil pollution status of agricultural land in Hebei Province.
- plant fruit,
- total nitrogen,
- concentrated sulfuric acid,
- graphite digestion apparatus,
- Kjeldahl method,
- automatic titration

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Determination of Total Nitrogen in Plant Fruits by Graphite Digestion Apparatus and Automatic Azotometer

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