Inter-Laboratory Comparison of Measuring Organic Carbon and Stable Nitrogen Isotopes in Marine Sediments by Elemental Analysis-Isotope Ratio Mass Spectrometry

CHANG Wen-bo; LI Feng; ZHANG Yuan-yuan; HE Xing-liang

doi:10.15898/j.cnki.11-2131/td.202003090027

Rock and Mineral Analysis > 2020 > 39(4): 535-545. > DOI: 10.15898/j.cnki.11-2131/td.202003090027

CHANG Wen-bo, LI Feng, ZHANG Yuan-yuan, HE Xing-liang. Inter-Laboratory Comparison of Measuring Organic Carbon and Stable Nitrogen Isotopes in Marine Sediments by Elemental Analysis-Isotope Ratio Mass Spectrometry[J]. Rock and Mineral Analysis, 2020, 39(4): 535-545. DOI: 10.15898/j.cnki.11-2131/td.202003090027

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Inter-Laboratory Comparison of Measuring Organic Carbon and Stable Nitrogen Isotopes in Marine Sediments by Elemental Analysis-Isotope Ratio Mass Spectrometry

Qingdao Institute of Marine Geology, Qingdao 266071, China

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Received Date: March 08, 2020
Revised Date: April 12, 2020
Accepted Date: June 01, 2020
Published Date: June 30, 2020

Abstract

Highlights
(1) The comparison of analytical results of organic carbon and nitrogen isotopes in marine sediments has been carried out in ten laboratories in China and good precision has been obtained.

(2) The applicability of EA-IRMS for the determination of organic carbon and nitrogen stable isotopes in marine sediments was verified.

(3) A reference for using EA-IRMS to determine organic carbon and nitrogen stable isotopes in marine sediments has been established.

ABSTRACT

BACKGROUNDOrganic carbon and nitrogen stable isotopes (δ¹³C, δ¹⁵N) in marine sediments are used as effective indicators to trace the changes of air, temperature, precipitation and other parameters in different geological time. Elemental analysis-isotope ratio mass spectrometry is a reasonable and effective method to measure the composition of organic carbon and nitrogen stable isotopes in marine sediments. At present, many domestic laboratories use the method of element analyzer (EA) and stable isotope ratio mass spectrometer (IRMS) to analyze the stable isotopes of organic carbon and nitrogen. However, the comparison of analytical method and data stability between different laboratories is lacking. The analytical results lack effective traceability, and the process lacks standardization and unification.
OBJECTIVESTo develop a method for determining organic carbon and nitrogen stable isotopes in marine sediments.
METHODSThree newly developed national first-class standard materials (GBW04701, GBW04702, GBW04703) and two international standard materials (EMA-B2152, EMA-B2151) were sent to 10 domestic laboratories, in order to verify the stability, precision and accuracy of EA-IRMS online technology test method through the comparative measurement between laboratories.
RESULTSThe data of each collaborative laboratory was accurate and stable, and the method had good repeatability and reproducibility. The results showed that precisions of ¹³C and ¹⁵N were better than 0.10‰ and 0.14‰, respectively.
CONCLUSIONSThrough comparative study, it has been proved that EA-IRMS online technology is suitable for the determination of organic carbon and nitrogen stable isotopes in marine sediments. A suitable method for the determination of marine sediment samples has been preliminarily established.

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References (30)

References

Molero G, Aranjuelo I, Teixidor P, et al.Measurement of ¹³C and ¹⁵N isotope labeling by gas chromatography/combustion/isotope ratio mass spectrometry to study amino acid fluxes in a plant-microbe symbiotic association[J].Rapid Communications in Mass Spectrometry, 2011, 25:599-607. doi: 10.1002/rcm.4895

张咏华, 吴自军.陆架边缘海沉积物有机碳矿化及其对海洋碳循环的影响[J].地球科学进展, 2019, 34(2):202-209. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=dqkxjz201902010

Zhang Y H, Wu Z J.Sedimentary organic carbon mineralization and its contribution to the marine carbon cycle in the marginal seas[J].Advances in Earth Science, 2019, 34(2):202-209. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=dqkxjz201902010

葛晨东, 王颖, Pedersen T F, 等.海南岛万泉河口沉积物有机碳、氮同位素的特征及其环境意义[J].第四纪研究, 2007, 27(5):845-852. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=dsjyj200705026

Ge C D, Wang Y, Pedersen T F, et al.Variability of organic carbon isotope, nitrogen isotope, and C/N in the Wanquan River Estuary, eastern Hainan Island, China and its environmental implications[J].Quaternary Sciences, 2007, 27(5):845-852. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=dsjyj200705026

Volvoikar S P, Nayak G N, Mazumdar A, et al.Recon-struction of depositional environment of a tropical estuary and response of δ¹³C_org and TOC/TN signatures to changing environmental conditions[J].Estuarine, Coastal and Shelf Science, 2014(139):137-147. https://www.sciencedirect.com/science/article/abs/pii/S0272771414000080

高艺珅, 王红亚, 程颖, 等.太白山西佛爷池沉积物有机碳、氮同位素记录的过去1800多年的气候-环境变化[J].北京大学学报(自然科学版), 2014, 35(5):1-11. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=bjdxxb201801010

Gao Y S, Wang H Y, Cheng Y, et al.Climatic and environmental changes in more than the past 1800 years as recorded by isotopic carbon and nitrogen in organic matter of sediments from Western Foye Chi (Buddha Pond) on Taibai Mountain[J].Acta Scientiarum Naturalium Universitatis Pekinensis, 2014, 35(5):1-11. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=bjdxxb201801010

Vaalgamaa S, Sonninen E, Korhola A, et al.Identifying recent sources of organic matter enrichment and eutrophication trends at coastal sites using stable nitrogen and carbon isotope ratios in sediment cores[J].Original Paper, 2013, 50:191-206. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=eeff38de40f6208cbc64d7a9243988dd

Sun W W, Shen J, Zhang E L, et al.Stable nitrogen isotope record of lacustrine sediments in Lake Onuma (northern Japan) indicates regional hydrological variability during the past four centuries[J].Quaternary International, 2016, 397:307-316. doi: 10.1016/j.quaint.2015.07.036

王毛兰, 艾永平, 张丁苓.鄱阳湖三江口柱状沉积物有机氮同位素特征及其环境指示意义[J].中国环境科学, 2015, 35(2):558-564. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zghjkx201502031

Wang M L, Ai Y P, Zhang D L.Stable organic nitrogen isotope in core sediments of the Three Rivers Estuary, Poyang Lake and its environmental implications[J].China Environmental Science, 2015, 35(2):558-564. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zghjkx201502031

Brodie C R, Leng M J, Casford J S L, et al.Evidence for bias in C and N concentrations and δ¹³C composition of terrestrial and aquatic organic materials due to pre-analysis acid preparation methods[J].Chemical Geology, 2011, 282:67-83. doi: 10.1016/j.chemgeo.2011.01.007

张凌, 陈繁荣, 殷克东, 等.珠江口及近海表层沉积有机质的特征和来源[J].环热带海洋学报, 2010, 29(1):98-103. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=rdhy201001015

Zhang L, Chen F R, Yin K D, et al.The characteristics and sources of surface sediments in the Pearl River Estuary and its adjacent shelves[J].Journal of Tropical Oceanoraphy, 2010, 29(1):98-103. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=rdhy201001015

王毛兰, 赖建平, 胡珂图, 等.鄱阳湖表层沉积物有机碳、氮同位素特征及其来源分析[J].中国环境科学, 2014, 34(4):1019-1025. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zghjkx201404042

Wang M L, Lai J P, Hu K T, et al.Compositions and sources of stable organic carbon and nitrogen isotopes in surface sediments of Poyang Lake[J].China Environmental Science, 2014, 34(4):1019-1025. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zghjkx201404042

冀文豪, 郭匿春, 徐军, 等.长江中游浅水湖泊沉积物碳氮同位素特征及其来源分析[J].水生态学杂志, 2018, 39(6):8-15. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=sstxzz201806002

Ji W H, Guo N C, Xu J, et al.Carbon and nitrogen isotope characterization and source analysis of sediments from shallow lakes in the middle of Yangtze River[J].Journal of Hydroecology, 2018, 39(6):8-15. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=sstxzz201806002

Mishraa S, Jhab N, Stebbinsc A, et al.Palaeoenvironments, flora, and organic carbon and nitrogen isotope changes across the non-marine Permian-Triassic boundary at Wybung Head, Australia[J].Palaeogeography, Palaeoclimatology, Palaeoecology, 2019, 534:1-21.

于广磊, 李斌, 李凡, 等.黄河口附近海域沉积物中碳氮元素地球化学特征及有机质来源研究[J].海洋环境科学, 2019, 38(6):862-867. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyhjkx201906007

Yu G L, Li B, Li F, et al.Carbon, nitrogen geochemical character and organic matter source study in the coastal sediment of Yellow River Estuary[J].Marine Environmental Science, 2019, 8(6):862-867. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyhjkx201906007

梁越, 肖化云, 刘小真, 等.δ¹³C和δ¹⁵N指示不同生态类型湖泊无机氮及有机质来源[J].湖泊科学, 2014, 26(5):691-697. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hpkx201405006

Liang Y, Xiao H Y, Liu X Z, et al.Identifying provenance of inorganic nitrogen and organic matter in different ecotype lakes using δ¹³C and δ¹⁵N[J].Journal of Lake Sciences, 2014, 26(5):691-697. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hpkx201405006

金贵善, 刘汉彬, 张建锋, 等.EA-IRMS测定有机及无机氮同位素条件探讨[J].地质学报, 2015, 89(增刊1):85-86. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=8771779

Jin G S, Liu H B, Zhang J F, et al.Discussion on the determination of organic and inorganic nitrogen isotopes with EA-IRMS[J].Acta Geological Sinica, 2015, 89(Supplement 1):85-86. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=8771779

孟宪菁, 杨斌, 李安, 等.具有动态稀释功能的EA-IRMS联机系统在δ¹³C和δ¹⁵N测定中的应用[J].质谱学报, 2018, 39(6):746-753. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zpxb201806012

Meng X J, Yang B, Li A, et al.Application of dynamic dilution on δ¹³C and δ¹⁵N measurements using EA-IRMS system[J].Journal of Chinese Mass Spectrometry Society, 2018, 39(6):746-753. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zpxb201806012

白亚之, 刘季花, 张辉, 等.海洋沉积物有机碳和总氮分析方法[J].海洋环境科学, 2013, 32(3):444-459. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyhjkx201303026

Bai Y Z, Liu J H, Zhang H, et al.The analysis of organic carbon and total nitrogen in marine sediments[J].Marine Environmental Science, 2013, 32(3):444-459. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyhjkx201303026

孙萱, 宋金明, 于颖, 等.元素分析仪快速测定海洋沉积物TOC和TN的条件优化[J].海洋科学, 2014, 38(7):14-19. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hykx201407003

Sun X, Song J M, Yu Y, et al.A rapid method for determing the total organic carbon and total nitrogen in marine sediments with an elemental analyzer[J].Marine Sciences, 2014, 38(7):14-19. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hykx201407003

秦德谛, 贺行良, 张媛媛, 等.渤海东海海洋沉积物中碳氮稳定同位素标准物质研制[J].岩矿测试, 2017, 36(1):857-862. doi: 10.15898/j.cnki.11-2131/td.2017.01.011

Qin D D, He X L, Zhang Y Y, et al.The preparation of carbon and nitrogen stable isotopes reference materials using sediments from the Bohai and East China Sea[J].Rock and Mineral Analysis, 2017, 36(1):857-862. doi: 10.15898/j.cnki.11-2131/td.2017.01.011

彭亚君, 王玉珏, 刘东艳, 等.酸化过程对海洋沉积物中有机碳同位素分析的影响[J].海洋学报, 2015, 37(12):85-92. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyxb201512009

Peng Y J, Wang Y J, Liu D Y, et al.Acid treatment effects on the carbon stable isotope values of marine sediments[J].Acta Oceanologica Sinica, 2015, 37(12):85-92. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyxb201512009

谭扬, 吴学丽, 侯立杰.样品处理方法对海洋沉积物有机碳稳定同位素测定的影响[J].海洋环境科学, 2017, 36(4):388-395. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyhjkx201805023

Tan Y, Wu X L, Hou L J.The effects of sample treatment methods on marine sediment organic carbon stable isotope[J].Marine Environmental Science, 2017, 36(4):388-395. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyhjkx201805023

曹建, 黄奕普, 刘广山, 等.海洋悬浮颗粒物中氮同位素的EA-IRMS法测定[J].台湾海峡, 2003, 22(1):1-8. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=twhx200301001

Cao J, Huang Y P, Liu G S, et al.Determination of ¹⁵N in marine suspended particulate matter using EA-IRMS technique[J].Journal of Oceanography in Taiwan Strait, 2003, 22(1):1-8. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=twhx200301001

Brodie C R, Casford J S L, Lloyd J M, et al.Evidence for bias in C/N, δ¹³C and δ¹⁵N values of bulk organic matter, and on environmental interpretation, from a lake sedimentary sequence by pre-analysis acid treatment methods[J].Quaternary Science Reviews, 2011, 30:3076-3087. doi: 10.1016/j.quascirev.2011.07.003

陈立雷, 张媛媛, 贺行良, 等.海洋沉积物有机碳和稳定氮同位素分析的前处理影响[J].沉积学报, 2014, 32(6):1046-1051. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=cjxb201406006

Chen L L, Zhang Y Y, He X L, et al.The research on sample-pretreatment of organic carbon and stable nitrogen isotopes in marine sediments[J].Acta Sedimentologica Sinica, 2014, 32(6):1046-1051. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=cjxb201406006

Gentile N, Rossi M J, Delémont O, et al.δ¹⁵N measure-ment of organic and inorganic substances by EA-IRMS:A speciation-dependent procedure[J].Analytical and Bioanalytical Chemistry, 2013, 405:159-176. doi: 10.1007/s00216-012-6471-z

张媛媛, 贺行良, 孙书文, 等.元素分析仪-同位素比值质谱仪测定海洋沉积物有机碳稳定同位素方法初探[J].岩矿测试, 2014, 32(6):1046-1051. http://www.ykcs.ac.cn/article/id/ykcs_20120413

Zhang Y Y, He X L, Sun S W, et al.A preliminary study on the determination of organic carbon stable isotope of marine sediment by element analyzer-isotope ratio mass spectrometer[J].Rock and Mineral Analysis, 2014, 32(6):1046-1051. http://www.ykcs.ac.cn/article/id/ykcs_20120413

徐丽, 邢蓝田, 王鑫, 等.元素分析仪-同位素比值质谱测量碳氮同位素比值最佳反应温度和进样量的确定[J].岩矿测试, 2018, 37(1):15-20. doi: 10.15898/j.cnki.11-2131/td.201701130005

Xu L, Xing L T, Wang X, et al.Study on the optimal reaction temperature and sampling weight for measurement of carbon and nitrogen isotope ratio by elemental analyzer-isotope ratio mass spectrometer[J].Rock and Mineral Analysis, 2018, 37(1):15-20. doi: 10.15898/j.cnki.11-2131/td.201701130005

蓝高勇, 吴夏, 杨会, 等.激光同位素光谱法测量水中氢氧同位素组成的实验室间比对研究[J].岩矿测试, 2017, 36(5):460-467. doi: 10.15898/j.cnki.11-2131/td.201704060049

Lan G Y, Wu X, Yang H, et al.Inter-laboratory comparison of analysis for hydrogen and oxygen stable isotope ratios in water samples by laser absorption spectroscopy[J].Rock and Mineral Analysis, 2017, 36(5):460-467. doi: 10.15898/j.cnki.11-2131/td.201704060049

王华, 吴夏, 蓝高勇, 等.GasBench Ⅱ-IRMS稳定同位素质谱法高精度测定环境水体中δD, δ¹⁸O和δ¹³C_DIC[J].地质学报, 2015, 89(10):1804-1813. http://www.cqvip.com/QK/95080X/201510/666332910.html

Wang H, Wu X, Lan G Y, et al.High precision measurement of hdrogen oxygen and dissolve inorganic carbon isotope in water samples by GasBenchⅡ-IRMS:An interlaboratory comparison study[J].Acta Geologica Sinica, 2015, 89(10):1804-1813. http://www.cqvip.com/QK/95080X/201510/666332910.html

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