Abstract: In 2011, new healthy standards for drinking water were implemented, which included 106 health indicators and related detection methods. In the national standard methods, microcystins and atrazine are detected by Liquid Chromatography with Ultraviolet Detector; acrylamide, bentazone and 2,4-D are detected by Gas Chromatography with Electron Capture Detector, carbofuran and glyphosate are measured by Liquid Chromatography with Fluorescence Detector. All of these methods are cumbersome and have poor operability making them difficult to conduct on a daily basis. A method for the analysis of 8 organic pollutants (glyphosate, carbofuran, bentazone, atrazine, 2,4-D, acrylamide, microcystin-RR and microcystin-LR) in drinking water, surface water and underground water was developed using Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry (UPLC-MS-MS). After filtration by 0.22 μm membrane, the water sample was injection directly into the equipment. Compared with recently reported methods, similar precision and accuracy were obtained and are presented in this paper. But shorter analysis time is needed and more target compounds are included. The result indicate that the LOQ of 8 compounds ranged from 0.07 to 5 μg/L, Meanwhile, the high correlation coefficients (r>0.993) of 8 compounds were obtained within the respective linear ranges. The average recoveries at low, intermediate and high spiked levels range from 96.7% to 101.7% with relative standard deviations (RSD, n=6) of 1.1%-3.5%. This method is suitable for the identification and quantification of the aforementioned 8 compounds in drinking water with the advantages of simple pretreatment, good reliability and high sensitivity.