Abstract: A new method for standard solution preparation was introduced, which utilized the sophisticated electronic balance as a quantitative tool in place of traditional quantitative tubes in standard solution preparation and dilution. Both the accuracy and repeatability by new method are good and without considering the influence factors, such as solution viscosity, on uncertainty. And the new method also greatly reduced the errors from scale reading and dilution operation etc. in gradual dilution of standard solutions. Based on Guide to Uncertainty Evaluation in Chemistry Analysis (CNAL/AG07:2002), the uncertainties from the new and traditional methods were evaluated. The accuracy and linearity of the new method were also tested by ICP-AES determination of analytes with satisfactory results. The method is suitable for the preparation of calibration solutions in determination of the major components with concentration range ofx%~xx% by ICP-AES and preparation of calibration solutions in AFS and AAS analysis.