Hg2+ -碘化物-番红花红T缔合体系褪色光度法测定微量汞
Fading Spectrophotometric Determination of Trace Mercury with Hg2+-Iodide-Safranine T Complex System
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摘要: 在0.01 mol/L 硫酸介质中,Hg2+与过量I-和番红花红T(ST)反应生成稳定的离子缔合物 2 ,使ST褪色,据此建立了褪色光度法测定微量汞的方法。最大褪色波长为519 nm,体系的褪色程度与Hg2+浓度在0~1.35 μg/mL内呈线性关系,表观摩尔吸光系数为1.24×105 L/(mol·cm),检出限为2.26 μg/L。采用巯基棉富集分离,测定河水中的微量汞,方法相对标准偏差(RSD,n=5)为1.2%~1.7%,回收率为98.3%~102.6%。Abstract: A fading spectrophotometric method for the determination of trace mercury has been developed. In 0.01 mol/L H2SO4 medium, Hg2+ reacts with excess I- and safranine T(ST) to form a stable ion-association complex ( 2 ), which leads to the fading of ST. The maximum fading wavelength locates at 519 nm. The fading degree of the system is linear with the concentration of Hg2+ in the range of 0~1.35 μg/mL. Apparent molar absorption coefficient is 1.24×105 L/(mol·cm) with detection limit of 2.26 μg/L for mercury. Recoveries of Hg2+ are 98.3%~102.6% with precision of 1.2%~1.7% RSD (n=5). The method has been applied in the determination of trace mercury in river water samples after enrichment and separation with thiol cotton fiber.
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Keywords:
- fading /
- spectrophotometry /
- mercury /
- iodide /
- safranine
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