气相色谱法测定土壤中的脂肪酸成分

Gas Chromatographic Analysis of the Fatty Acid Components in Soil Samples

摘要: 用气相色谱-质谱联用仪对脂肪酸进行定性,气相色谱配合标准溶液进行定量,建立了一种完整的测定土壤中脂肪酸含量的方法。方法应用于实际样品的检测,各组分的线性相关系数为0.9913~0.9999,仪器精密度(RSD,n=6)为0.73%~2.57%,检出限为0.016~0.038 mg/L,平均回收率为97.86%。建立的方法能够较准确地测定土壤中各种脂肪酸的含量,且可以满足实际样品的测定需要。

Abstract: A method for the determination of components in fatty acid in soil samples was established. Fatty acid components in the samples were separated and identified by gas chromatography-mass spectrometry (GC-MS) and each component was quantified by GC with standard solution calibration. The linear correlation coefficients (R²) of the method for each component were 0.9913~0.9999. The detection limits of the method for the components were 0.016~0.038 mg/L. Average recovery of the method was 97.86% with precision of 0.73%~2.57%RSD(n=6). The method has been applied to the quantitative analysis of fatty acid components in soil samples with satisfactory results.