Abstract: The Qingshiling tungsten-polymetallic deposit field in Hunan province is a new discovered metallogenic region with great potential of mineral prospecion. Based on the previous research progress in this region, Re-Os isotopic age of molybdenite from fracture zonealterated rock type of tungsten-polymetallic deposit in Zhuguangshan complex granite rock-body. The isochron age of (147.6±6.8) Ma was obtained, which suggests its mineralization is related to the Early Yanshanian granite intrusive activities.