土壤和白菜中低分子量有机酸的气相色谱分析

Determination of Low Molecular Weight Organic Acids in Soils and Cabbages by Gas Chromatography

摘要: 利用甲醇-硫酸混合液（体积比10∶7）对土壤和白菜中低分子量有机酸同时进行提取和甲酯化衍生，然后利用二氯甲烷液-液萃取，气相色谱法分析。土壤中7种低分子量有机酸（草酸、丙二酸、反丁烯二酸、琥珀酸、马来酸、L-苹果酸、柠檬酸）的添加回收率为81.93%～95.58％，白菜中的添加回收率为84.78％～106.99％，方法的检出限为0.72～4.14 mg/kg。方法用于测定根际、非根际土壤和白菜根系、茎叶中的低分子量有机酸，结果准确可靠。

Abstract: The low molecular weight organic acids (LMWOAs) in rhizosphere soil, bulk soil, root and leave of cabbages were extracted and derived by the mixture of methanol and sulfuric acid (10∶7). Seven organic acids (oxalic, malonic, fumaric, succinic, maleic, L-malic and citric acid) were identified and quantified by GC instrument equipped with FID. The recovery were ranged from 81.93% to 95.58% for soils and from 84.78% to 106.99% for roots. The detection limit was ranged from 0.72 mg/kg to 4.14 mg/kg. The method was suitable for the reliable and accurate determination of low molecular weight organic acids in soils and cabbages.