Abstract: A method for the determination of C37 alkenones by high pressure gas chromatography using chromatographic column with high separation speed is proposed in this paper. The influences of carrier gas pressure and temperature programming rate on the determination results were studied. Under the condition of the temperature programming from 80℃ to 280℃ at the rate of 20 ℃/min and the head pressure of 550 kPa, the complete separation of C37 alkenones including C37:2, C37:3, C37:4 can be obtained and the period of the instrumental analysis is reduced to 40 min. The detection limit of the method is 10 ng/mL for long-chain alkenones with precision of less than 4%RSD (n=13).