Abstract: Helium is an indispensable and rare strategic element. A method of sampling the top helium gas in the helium gas resource investigation was used, and is described in this paper. Thermal conductivity detector (TCD) of the Gas Chromatograph was used to detect helium gas. The helium gas standard was used to study the helium gas storage container, the storage medium, the storage temperature, and the storage time. Results show that the glass jar, the saturated salt water, the low temperature, and the inverted storage are the best methods to save the head space gas. At room temperature, the helium head space gas storage period is about 2 months, whereas under refrigerated conditions (4℃), the storage time reaches 3 months. Therefore, the author suggests that the helium head space gas collection method is as follows. The fresh soil samples are collected in a 500 mL glass bottle, and then are injected into the saturated salt water. A 50 mL space remains in the bottle, and then the bottle is sealed by silica gel and kept inverted. For the helium top gas stored under normal temperature and refrigerated conditions, the test should be completed within the corresponding storage period to ensure the accuracy of the test data.